群馬大学 大学院保健学研究科 医学部保健学科

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保健学科について / 保健学研究科について

プロフィール 教育と研究 臨床業務、地域・国際貢献 業 績

▌プロフィール

大西 浩史
おおにしひろし 大西 浩史 Hiroshi Ohnishi 基礎保健学ユニット 生体情報検査学領域 E-mail website
現 職
群馬大学医学部保健学科教授
群馬大学大学院保健学研究科教授
所属学会
日本神経化学会,日本神経科学学会,日本生化学会,日本分子生物学会
日本プロテインホスファターゼ研究会,日本癌学会,北米神経科学会
コメント
神経機能や生体のストレス応答を対象に、生体制御に関わる細胞シグナルの分子メカニズムと機能について基礎研究を行っています。生命システムの姿を明らかにすることで、より健康で安全な暮らしの基礎を形づくることを目指します。

▌教育と研究

研究内容

生体情報の伝達・制御に関わるシグナル分子の作用メカニズムを解析し、それらが健康と疾患へどのように関与するのかを理解することを目標に研究を行う。

・行動を制御する脳内分子シグナルについての研究

・脳内免疫システムについての研究

・生体の低温ストレス応答および冬眠制御機構についての研究

教育業務

学部教育

学部教育:分子生物学、遺伝子工学、薬理学、遺伝子検査学実習 など

教養教育:化学、基礎分子生物学 など

 

大学院教育

博士前期課程:基礎保健学ユニット生体情報検査科学分野

分子情報分析科学特論、分子情報分析科学演習、

生体情報検査科学特別セミナー、分子情報分析科学特別研究Ⅱ

博士後期課程:生体情報検査科学領域 基礎生体情報検査科学分野

分子情報分析科学特講、分子情報分析科学演習、基礎情報分析科学特別研究

▌臨床業務、地域・国際貢献

地域・国際貢献
  • 日本神経化学会 評議員
  • 日本プロテインホスファターゼ研究会 世話人
  • まちなかキャンパス 主催:群馬大学生体調節研究所・前橋商工会議所(2009-2011)
  • 世界脳週間2009「脳の仕組みと働きを考える」 主催:NPO法人 脳の世紀推進会議、群馬大学神経科学グループ(2009)
  • 理科体験教室「群馬おもしろ科学展」主催:群馬大学(2006)

▌業 績

  1. Koshimizu, H, Takao, K, Matozaki, T, Ohnishi, H, Miyakawa, T : Comprehensive behavioral analysis of Cluster of Differentiation 47 knocout mice. PLoS ONE 9(2): e89584., 2014
  2. Murata,Y., Saito, Y., Kaneko, T., Kotani, T., Kaneko, Y., Ohnishi, H., Matozaki, T. : Autoimmune animal models in the analysis of the CD47-SIRPα signaling pathway. Methods 65: 254-259, 2014
  3. Zen, K., Guo, Y., Bian, Z., Lv, Z., Zhu, D., Ohnishi, H., Matozaki, T., Liu, Y. : Inflammation-induced proteolytic processing of the SIRPα cytoplasmic ITIM in neutrophils propagates a proinflammatory state. Nat Commun 4: 2436, 2013
  4. Takahashi, S., Tomioka, M., Hiromura, K., Sakairi, T., Hamatani, H., Watanabe, M., Ikeuchi, H., Kaneko, Y., Maeshima, A., Aoki, T., Ohnishi, H., Matozaki, T., Nojima, Y. : SIRPα signaling regulates podocyte structure and function. Am J Physiol Renal Physiol 305: F861-870, 2013
  5. Hayashi, Y., Kusakari, S., Sato-Hashimoto, M., Urano, E., Shigeno, M., Sekijima, T., Kotani, K., Murata, Y., Murakami, H., Matozaki, T., Ohnishi, H. : Hypothermia-dependent and -independent effects of forced swim on the phosphorylation states of signaling molecules in mouse hippocampus. Biochem Biophys Res Commun 428: 475-481, 2012
  6. Maruyama, T., Kusakari, S., Sato-Hashimoto, M., Hayashi, Y., Kotani, K., Murata, Y., Okazawa, H., Oldenborg, P.-A., Kishi, S., Matozaki, T., Ohnishi, H. : Hypothermia-induced tyrosine phosphorylation of SIRPα in the brain. J Neurochem 121: 891-902, 2012
  7. Kaneko, T., Saito, Y., Kotani, T., Okazawa, H., Iwamura, H., Sato-Hashimoto, M., Kanazawa, Y., Takahashi, S., Hiromura, K., Kusakari, S., Kaneko, Y., Murata, Y., Ohnishi, H., Nojima, Y., Takagishi, K., Matozaki, T. : Dendritic cell-specific ablation of the protein tyrosine phosphatase Shp1 promotes Th1 cell differentiation and induces autoimmunity. J Immunol 188: 5397-5407, 2012
  8. Nakakura, T., Mogi, C., Tobo, M., Tomura, H., Sato, K., Kobayashi, M., Ohnishi, H., Tanaka, S., Wayama, M., Sugiyama, T., Kitamura, T., Harada, A., Okajima, F. : Deficiency of proton-sensing ovarian cancer G protein-coupled receptor 1 attenuates glucose-stimulated insulin secretion. Endocrinology 153: 4171-4180, 2012.
  9. Wang, L., Lu, Y., Deng, S., Zhang, Y., Yang, L., Guan, Y., Matozaki, T., Ohnishi, H., Jiang, H., Li, H. : SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress. J Neurochem 122: 834-843, 2012
  10. Ohnishi, H., Murata, Y., Okazawa, H., Matozaki, T. : Src family kinases: Modulators of neurotransmitter receptor function and behavior. Trends Neurosci 34: 629-637, 2011
  11. Sato-Hashimoto, M., Saito, Y., Ohnishi, H., Iwamura, H., Kanazawa, Y., Kaneko, T., Kusakari, S., Kotani, T., Mori, M., Murata, Y., Okazwa, H., Ware, C. F., Oldenborg, P.-A., Nojima, Y., Matozaki, T. : SIRPα regulates the homeostasis of T lymphocytes in the spleen. J Immunol 187: 291-297, 2011
  12. Iwamura, H., Saito, Y., Sato-Hashimoto, M., Ohnishi, H., Murata, Y., Okazawa, H., Kanazawa, Y., Kaneko, T., Kusakari, S., Kotani, T., Nojima, Y., Matozaki, T. : Essential roles of SIRPα in homeostatic regulation of skin dendritic cells. Immunol Lett 135(1-2): 100-107, 2011
  13. Kanazawa, Y., Saito, Y., upriatna, Y., Tezuka, H., Kotani, T., Murata, Y., Okazawa, H., Ohnishi, H., Kinouchi, Y., Nojima, Y., Ohteki, T., Shimosegawa, T., Matozaki, T. : Role of SIRPα in regulation of mucosal immunity in the intestine. Genes Cells 15, 1189-1200, 2010
  14. Morimoto, N., Murata, Y., Motegi, S., Suzue, K., Saito, Y., Okazawa, H., Ohnishi, H., Kotani, T., Kusakari, S., Ishikawa, O., Matozaki, T. : Requirement of SIRPα for protective immunity against Leishmania major. Biochem Biophys Res Commun 401, 385-389, 2010
  15. Ohnishi, H., Murata, T., Kusakari, S., Hayashi, Y., Takao, T., Maruyama, T., Ago, Y., Koda, K., Jin, F.-J., Okawa, K., Oldenborg, P.-A., Okazawa, H., Murata, Y., Furuya, N., Matsuda, T., Miyakawa, T., Matozaki, T. : Stress-evoked tyrosine phosphorylation of SIRPα regulates behavioral immobility in the forced swim test. J Neurosci 30: 10472-10483, 2010
  16. Saito, Y., Iwamura, H., Kaneko, T., Ohnishi, H., Murata, Y., Okazawa, H., Kanazawa, Y., Sato-Hashimoto, M., Kobayashi, H., Oldenborg, P.-A., Naito, M., Kaneko, Y., Nojima, Y., Matozaki, M. : Regulation by SIRPα of dendritic cell homeostasis in lymphoid tissues.Blood 116: 3517-3525, 2010
  17. Matozaki, T., Murata, Y., Saito, Y., Okazawa, H., Ohnishi, H. : Protein tyrosine phosphatase SHP-2: A proto-oncogene product that promotes Ras activation. Cancer Sci 100: 1786-1793, 2009
  18. Matozaki, T., Murata, Y., Okazawa, H., Ohnishi, H. : Functions and molecular mechanisms of the CD47-SIRPα signalling pathway. Trends Cell Biol 19: 72-80, 2009
  19. Kobayashi, M., Ohnishi, H., Okazawa, H., Murata, Y., Hayashi, Y., Kobayashi, H., Kitamura, T., Matozaki, T. : Expression of SHPS-1 in pancreatic β-cells and its role in promotion of insulin secretion and protection against diabetes. Endocrinology 149: 5662-5669, 2008
  20. Motegi, S., Okazawa, H., Murata, Y., Kanazawa, Y., Saito, Y., Kobayashi, H., Ohnishi, H., Oldenborg, P.-A., Ishikawa, O., Matozaki, T. : Essential roles of SHPS-1 in induction of contact hypersensitivity of skin. Immunol Lett 121: 52-60, 2008
  21. Okuzawa, C., Kaneko, Y., Murata, Y., Miyake, A., Saito, Y., Okajo, J., Tomizawa, T., Kaneko, Y., Okazawa, H., Ohnishi, H., Matozaki, T., Nojima, Y. : Resistance to collagen-induced arthritis in SHPS-1 mutant mice. Biochem Biophys Res Commun 371: 561-566, 2008
  22. Kusakari, S., Ohnishi, H., Jin, F.-J., Kaneko, Y., Murata, T., Murata, Y., Okazawa, H., Matozaki, T. : Trans-endocytosis of CD47 and SHPS-1 and its role in regulation of the CD47-SHPS-1 system. J Cell Sci 121: 1213-1223, 2008
  23. Kaneko, Y., Kaneko, Y., Ohnishi, H., Tomizawa, T., Okajo, J., Saito, Y., Okuzawa, C., Murata, Y., Okazawa, H., Nojima, Y., Okamoto, K., Matozaki, T. : Impaired proliferation and Th1 differentiation of CD4+ T cells of SHPS-1 mutant mice. Kitakanto Med J 58: 133-139, 2008
  24. Miyake, A., Murata, Y., Okazawa, H., Ikeda, H., Niwayama, Y., Ohnishi, H., Hirata, Y., Matozaki, T. : Negative regulation by SHPS-1 of Toll-like receptor-dependent proinflammatory cytokine production in macrophages. Genes Cells 13: 209-219, 2008
  25. Nakaishi, A., Hirose, M., Yoshimura, M., Oneyama, C., Saito, K., Kuki, N., Matsuda, M., Honma, N., Ohnishi, H., Matozaki, T., Masato Okada, M., Atsushi Nakagawa, A. : Structural insight into the specific interaction between murine SHPS-1/SIRPα and its ligand CD47. J Mol Biol 375: 650-660, 2008
  26. Tomizawa, T., Kaneko, Y., Kaneko, Y., Saito, Y., Ohnishi, H., Okajo, J., Okuzawa, C., Ishikawa-Sekigami, T., Murata, Y., Okazawa, H., Okamoto, K., Nojima, Y., Matozaki, T. : Resistance to experimental autoimmune encephalomyelitis and impaired T cell priming by dendritic cells in src homology 2 domain-containing protein tyrosine phosphatase substrate-1 mutant mice. J Immunol 179: 869-877, 2007
  27. Okajo, J., Kaneko, Y., Murata, Y., Tomizawa, T., Okuzawa, C., Saito, Y., Kaneko, Y., Ishikawa-Sekigami, T., Okazawa, H., Ohnishi, H., Matozaki, T., Nojima, Y. : Regulation by src homology 2 domain-containing protein tyrosine phosphatase substrate-1 of α-Galactosylceramide-induced antimetastatic activity and Th1 and Th2 responses of NKT cells. J Immunol 178: 6164-6172, 2007
  28. Murata, T., Ohnishi, H., Okazawa, H., Murata, Y., Kusakari, S., Hayashi, Y., Miyashita, M., Itoh, H., Oldenborg, P.-A., Furuya, N., Matozaki, T. : CD47 promotes neuronal development through Src- and FRG/Vav2-mediated activation of Rac and Cdc42. J Neurosci 26: 12397-12407, 2006
  29. Ikeda, H., Okazawa, H., Ohnishi, H., Murata, Y., Oldenborg, P.-A., Matozaki, T. : Mutational analysis of the mechanism of negative regulation by SRC homology 2 domain-containing protein tyrosine phosphatase substrate-1 of phagocytosis in macrophages. J Immunol 177: 3123-3132, 2006
  30. Shinohara, M., Ohyama, N., Murata, Y., Okazawa, H., Ohnishi, H., Ishikawa, O., Matozaki, T. : CD47 regulation of epithelial cell spreading and migration, and its signal transduction. Cancer Sci 97: 889-895, 2006
  31. Ishikawa-Sekigami, T., Kaneko, Y., Saito, Y., Murata, Y., Okazawa, H., Ohnishi, H., Oldenborg, P.-A., Nojima, Y., Matozaki, T. Enhanced phagocytosis of CD47-deficient red blood cells by splenic macrophages requires SHPS-1. Biochem Biophys Res Commun 343: 1197-1200, 2006
  32. Nagata, A., Ohnishi, H., Yoshimura, M., Ogawa, A., Ujita, S., Adachi, H., Okada, M., Matozaki, T., Nakagawa, A. Crystallization and preliminary X-ray analysis of rat SHPS-1. Acta Crystallogr Sect F Struct Biol Cryst Commun 62: 189-191, 2006
  33. Ishikawa-Sekigami, T., Kaneko, Y., Okazawa, H., Tomizawa, T., Okajo, J., Saito, Y., Okuzawa, C., Sugawara-Yokoo, M., Nishiyama, U., Ohnishi, H., Matozaki, H., Nojima, Y. : SHPS-1 promotes the survival of circulating erythrocytes through inhibition of phagocytosis by splenic macrophages. Blood 107: 341-348, 2006
  34. Ohnishi, H., Kaneko, Y., Okazawa, H., Miyashita, M., Sato, R., Hayashi, A., Tada, K., Nagata, S., Takahashi, M., Matozaki, T. : Differential localization of SH2 domain-containing protein tyrosine phosphatase substrate-1 and CD47 and its molecular mechanism in cultured hippocampal neurons. J Neurosci 25: 2702-2711, 2005
  35. Okazawa, H., Motegi, S., Ohyama, N., Ohnishi, H., Tomizawa, T., Kaneko, Y., Oldenborg, P.-A., Ishikawa, O., Matozaki, T. : Negative regulation of phagocytosis in macrophages by the CD47-SHPS-1 system. J Immunol 174: 2004-2011, 2005
  36. Miyashita, M., Ohnishi, H., Okazawa, H., Tomonaga, H., Hayashi, A., Fujimoto, T.-T., Nobuhiko Furuya, N., Matozaki, T. : Promotion of neurite and filopodium formation by CD47: Roles of integrins, Rac, and Cdc42. Mol Biol Cell 15: 3950-3963, 2004
  37. Hayashi, A. Ohnishi, H., Okazawa, H., Nakazawa, S., Ikeda, H., Motegi, S., Aoki, N., Kimura, S., Mikuni, M., Matozaki, T. : Positive regulation of phagocytosis by SIRPβ and its signaling mechanism in macrophages. J Biol Chem 279: 29450-29460, 2004
  38. Ohnishi, H., Kobayashi, H., Okazawa, H., Ohe, Y., Tomizawa, K., Sato, R., Matozaki, T. : Ectodomain shedding of SHPS-1 and its role in regulation of cell migration. J Biol Chem 279: 27878-27887, 2004
  39. Koga, T., Inui, M., Inoue, K., Kim, S., Suematsu, A., Kobayashi, E., Iwata, T., Ohnishi, H., Matozaki, T., Kodama, T., Taniguchi, T., Takayanagi, H., Takai, T. : Costimulatory signals mediated by the ITAM motif cooperate with RANKL for bone homeostasis. Nature 428: 758-763, 2004
  40. Murai-Takebe, R., Noguchi, T., Ogura, T., Mikami, T., Yanagi, K., Inagaki, K., Ohnishi, H., Matozaki, H., Kasuga, M. : Ubiquitination-mediated regulation of biosynthesis of the adhesion receptor SHPS-1 in response to endoplasmic reticulum stress. J Biol Chem 279: 11616-11625, 2004
  41. Sato, R., Ohnishi, H., Kobayashi, H., Kiuchi, D., Hayashi, A., Kaneko, Y., Honma, N., Okazawa, H., Hirata, Y., Matozaki, T. : Regulation of multiple functions of SHPS-1, a transmembrane glycoprotein, by its cytoplasmic region. Biochem Biophys Res Commun 309: 584-590, 2003
  42. Motegi, S., Okazawa, H., Ohnishi, H., Sato, R., Kaneko, Y., Kobayashi, H., Tomizawa, K., Ito, T., Honma, N., Bühring, H.-J., Ishikawa, O., Matozaki, T. : Role of the CD47-SHPS-1 system in regulation of cell migration. EMBO J 22: 2634-2644, 2003
  43. Takahashi, M., Ohnishi, H. : Negative regulation of exocytosis at the nerve terminal. Mol Psychiatry 7: 536-537, 2002
  44. Amino, S., Itakura, M., Ohnishi, H., Tsujimura, J., Koizumi, S., Takei, N., Takahashi, M. : Nerve growth factor enhances neurotransmitter release from PC12 cells by increasing Ca(2+)-responsible secretory vesicles through the activation of mitogen-activated protein kinase and phosphatidylinositol 3-kinase. J Biochem (Tokyo) 131: 887-894, 2002
  45. Yamasaki, S., Nishida, K., Hibi, M., Sakuma, M., Shiina, R., Takeuchi, A., Ohnishi, H., Hirano, T., Saito, T. : Docking protein Gab2 is phosphorylated by ZAP-70 and negatively regulates T cell receptor signaling by recruitment of inhibitory molecules. J Biol Chem 276: 45175-45183, 2001
  46. Hiroshi Ohnishi, Saori Yamamori, Kanako Ono, Kyota Aoyagi, Shunzo Kondo, Masami Takahashi. A src family tyrosine kinase inhibits neurotransmitter release from neuronal cells. Proc Natl Acad Sci, USA 98, 10930-10935, 2001
  47. Toshiyuki Araki, Masashi Yamada, Hiroshi Ohnishi, Shin-ichiro Sano, Hiroshi Hatanaka. BIT/SHPS-1 enhances brain-derived neurotrophic factor-promoted neuronal survival in cultured cerebral cortical neurons. J Neurochem 75, 1502-1510, 2000
  48. Toshiyuki Araki, Masashi Yamada, Hiroshi Ohnishi, Shin-ichiro Sano, Taichi Uetsuki, Hiroshi Hatanaka. Shp-2 specifically regulated several tyrosine-phosphorylated proteins in brain-derived neurotrophic factor signaling in cultured cerebral cortical neurons. J Neurochem 74, 659-668, 2000
  49. Ohnishi, H., Yamada, M., Kubota, M., Hatanaka, H., Sano, S. : Tyrosine phosphorylation and association of BIT with SHP-2 induced by neurotrophins. J Neurochem 72: 1402-1408, 1999
  50. Yamada, M., Ohnishi, H., Sano, S., Nakatani, A., Ikeuchi, T., Hatanaka, H. : Brain-derived neurotrophic factor stimulates interaction of Shp2 with PI3-K and Grb2 in cultured cerebral cortical neurons. J Neurochem 73: 41-49, 1999
  51. Sano, S., Ohnishi, H., Kubota, M. : Gene structure of mouse BIT/SHPS-1. Biochem J 344: 667-675, 1999
  52. Ohnishi, H., Kubota, M., Sano, S. : BIT (Bit) maps to mouse chromosome 2. Genomics 40, 504-506, 1997
  53. Yamada, M., Ohnishi, H., Sano, S., Nakatani, A., Ikeuchi, T., Hatanaka, H. : Insulin receptor substrate (IRS)-1 and IRS-2 are tyrosine-phosphorylated and associated with phosphatidylinositol 3-kinase in response to brain- derived neurotrophic factor in cultured cerebral cortical neurons. J Biol Chem 272: 30334-30339, 1997
  54. Sano, S., Ohnishi, H., Omori, A., Hasegawa, J., Kubota, M. : BIT, an immune antigen receptor-like molecule in the brain. FEBS Lett 411: 327-334, 1997
  55. Ohnishi, H., Sato, K., Ohtake, A., Kubota, M., Sano, S. : Activation of protein tyrosine phosphatase SH-PTP2 by a tyrosine-based activation motif of a novel brain molecule. J Biol Chem 271: 25569-25574, 1996